Recombinant Human Axl Kinase Protein (His Tag)

Recombinant Human Axl Kinase Protein (His Tag)

SKU #	PKSH031712
Expression Host	HEK293 Cells

Description

Synonyms	ARK,JTK11,Tyro7,UFO
Species	Human
Expression Host	HEK293 Cells
Sequence	Met 1-Pro 449
Accession	AAB20305.1
Calculated Molecular Weight	46.5 kDa
Observed Molecular Weight	60-70 kDa
Tag	C-His
Bio-activity	Label Recombinant Mouse GAS6 Protein (His Tag) with biotin. Immobilized Recombinant HumanAxl Kinase Protein (ECD, His Tag) at 0.25 g/mL (100 L/well) can bind biotinylated Recombinant Mouse GAS6 Protein, the EC50 is 1.3-3.9 ng/mL.

Properties

Purity	> 98 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background
Axl receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. Axl functions in dampening the immune response, regulating cytokine secretion, clearing apoptotic cells and debris, and maintaining cell survival. Axl is upregulated in various disease states, such as in the cuprizone toxicity-induced model of demyelination and in multiple sclerosis (MS) lesions, suggesting that it plays a role in disease pathogenesis. Axl expression correlates with poor prognosis in several cancers. Axl mediates multiple oncogenic phenotypes and activation of these RTKs constitutes a mechanism of chemoresistance in a variety of solid tumors. Axl contributes to cell survival, migration, invasion, metastasis and chemosensitivity justify further investigation of Axl as novel therapeutic targets in cancer. The receptor tyrosine kinase AXL is thought to play a role in metastasis. The soluble AXL receptor as a therapeutic candidate agent for treatment of metastatic ovarian cancer. GAS6/AXL targeting as an effective strategy for inhibition of metastatic tumor progression in vivo.